Fig. 6.

Pharmacological inhibition of ROCK mimics the effects of PKA.

Panel A: LNCaP cells were incubated with either vehicle (0.5% (v/v) DMSO) or 5 μM of the ROCK-selective inhibitor Y27632 for 1 h prior to stimulation with either vehicle (0.1% (v/v) EtOH) or 10 μM Fsk for 1 h. Phase contrast images of LNCaP cells were captured at 0 h and immediately following stimulation.

Panel B: Mean dendrite length determined (panel B). Results are presented as mean values ± SEM for n = 3 separate experiments. ***p < 0.001 vs. 0 h, ###p < 0.001 vs. vehicle at same time point, +++p < 0.001 vs. Fsk at 1 h.