Figure 4.
Sporozoite meutralizing activity in murine immune serum. Sera from (A) Balb/c or (B) C57Bl /6 mice immunized with three doses of (NANP)6-OMPC/MAA were tested in TSNA. PfPb sporozoites, that express P. falciparum CS repeats, were incubated with immune or control sera (1:5 dilution) prior to addition to HepG2 cells. Levels of parasites in HepG2 cell extracts collected after 48 h of culture were measured by qPCR. Results are shown as mean number of parasite 18S rRNA copies in cell extracts, based on an 18S rRNA plasmid standard. (C) Individual sera of 10 C57Bl/6 mice were tested in TSNA at dilutions of 1:10–1:40. Dotted line indicates 90% inhibition level.