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. 2012 Nov 9;48(3):329–342. doi: 10.1016/j.molcel.2012.08.024

Figure 6.

Figure 6

Residues in LC3C Providing Specificity for NDP52

(A) Superposition of LC3C (yellow) and LC3B (green), with residues lining the CLIR interaction groove in ball-and-stick representation.

(B) LUMIER binding assay. Normalized ratio between luciferase activity bound to beads and present in lysates. Lysates of 293ET cells expressing the indicated luciferase fusion constructs, incubated with purified GST-tagged proteins bound to beads.

(C–D) Superposition of NDP52 (blue), LC3C (yellow), LC3B (green) and GABARAP (red) with residues mediating the interaction in ball-and-stick representation. Enlarged: movement of the α2-helix in LC3B and GABARAP compared to LC3C.

(E) Percentage of bacteria coated by LC3B in HeLa cells stably expressing GFP-tagged LC3B. Cells transduced with mock, LC3A or the indicated LC3C alleles and treated with siRNA against LC3C were infected with S. Typhimurium for 1 hr. LC3A and LC3C mRNA levels are further characterized in Figure S6C. (All panels) Mean and s.d. of triplicate cultures. ∗p < 0.05, Student's t test.