FIG. 3.

Expression pattern of pluripotent markers in developmental stages of parthenotes. Lane 1, 100-bp marker; lane 2, immature oocytes; lane 3, mature oocytes; lane 4, two-cell; lane 5, four-cell; lane 6, eight- to 16-cell; lane 7, morula; lane 8, blastocyst; lane 9, negative RT; lane 10, negative PCR. A negative RT control was included in the reaction, which consisted of all of the components of the cDNA synthesis excluding the reverse transcriptase enzyme given in the kit. The negative PCR included all the components for PCR reaction except the template. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.