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. 2012 Dec;14(6):530–538. doi: 10.1089/cell.2012.0032

FIG. 7.

FIG. 7.

In vitro differentiation of PGESCs. (A) Neuron-like cells. (B) Characterization of neuron-like cells by NF-68 gene expression. Lane 1, 100-bp marker; lane 2, neuron-like cells; lane 3, negative RT; lane 4, negative PCR. (C) Hoechst staining of neuron cells. (D) Immunostaining of neuron cells by NESTIN. (E) Epithelial-like cells. (F) Characterization of epithelial-like cells by CYTOKARATIN 18 gene expression. Lane 1, 100-bp marker; lane 2, epithelial-like cells; lane 3, negative RT; lane 4, negative PCR. Scale bar, 50 μm.