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. 2012 Nov 30;2:905. doi: 10.1038/srep00905

Figure 3. SDS page image showing specific binding of cell protein to ganoderic acid DM (1) fixed with magnetic beads.

Figure 3

(A) Diagrams for ganoderic acid DM (1) fixation to the magnetic beads by reaction and amidation of the carboxylic group in the side chain of 1. (B) Lane 1: protein marker; lane 2: cytosolic protein incubated with FG beads; lane 3: cytosolic protein incubated with 1 (0.4 mM) bound FG beads; lane 4: cytosolic protein incubated with 1 (2 mM) bound FG beads; lane 5: cytosolic protein incubated with 1 (10 mM) bound FG beads. A specific binding protein of 46–58 kDa emerges with increased concentration of 1.