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. 2012 Sep 26;40(21):11163–11172. doi: 10.1093/nar/gks875

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — TALER fusion orientation. (A) Cartoon illustrating the split β-lactamase system used to evaluate TALER activity. (B) Schematic showing the fusion orientation of each TALER and its corresponding target site. (C) Activity of each designed TALER fusion against its intended DNA target. Recombination was normalized to background (vector only control). (D) Gin-Avr activity against cognate (Avr-20G) and non-cognate (Avr-20T, Avr-20GG, PthXo1-20G) DNA targets. Error bars indicate standard deviation (s.d.) (n = 3).