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. 2012 Sep 12;40(21):10866–10877. doi: 10.1093/nar/gks859

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Pre-existing homology is not required for class B product formation. (A) Structure of Zorro3 donor and view of Zorro3 regions where most class B junctions occur. Class B-I circles typically form from a joining between the 3′ poly(A) region and the 5′ poly(A) region, whereas class B-II circles typically form from a joining between the 3′ poly(A) region and the interORF poly(A) region. Zorro3pA⁻ sequences are identical to Zorro3 except the stretch of 20 (A)s in the 5′ poly(A) region is deleted. (B–E) Sequences of cloned class B product junctions derived from wild-type Zorro3 or Zorro3pA⁻ donors. Untemplated nucleotides are indicated in green. Yeast genome sequences are indicated in brown.