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. 2012 Sep 23;40(21):10916–10924. doi: 10.1093/nar/gks876

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Endonuclease assay for DNA cleavage by MSFusion. Final concentration of DNA (unmethylated pBRsk1) was 3 nM. Final concentration of reconstituted nuclease was 60 nM using at least 2 HsdR and 1 HsdM per M₁S₁ protein or MSFusion protein. The enzymes were reconstituted at 1000 nM and then diluted into the solution containing DNA. M = 1 kb ladder; OC = open circle; L = linear; CCC = covalent closed circle.