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. 2012 Sep 23;40(21):11100–11114. doi: 10.1093/nar/gks867

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Polysome association of Rbg1 mutants. Polysomes extracts were prepared from cells expressing TAP-tagged Tma46 and HA-tagged Rbg1 (wild-type or domain deletion mutants). Polysomes were resolved by density sedimentation in 10–50% sucrose gradient. The UV absorbance trace (254 nm) obtained by continuous monitoring during fractionation is shown with the position of the 40S, 60S, 80S and polysomes peaks indicated. Fractions were analyzed by western blotting to detect the TAP and HA tags. (A) Distribution of Tma46-TAP and wild-type HA-Rbg1. (B) Distribution of Tma46-TAP and HA-Rbg1 ΔTGS. (C) Distribution of Tma46-TAP and HA-Rbg1 ΔS5D2L. (D) Distribution of Tma46-TAP and HA-Rbg1 ΔHTH. Previously reported control analyses demonstrate that Rbg1 association with polysome is specific (15).