Figure 1.

DNA-binding properties of M. smegmatis and E. coli DNA gyrase. EMSA carried out with increasing concentrations of MsGyr (M. smegmatis) or EcGyr (E. coli) gyrase with radioactively labelled DNA molecules of different lengths. DNA binding with MsGyr (A–D) and EcGyr (E–H). The reactions were carried out at 25°C and then resolved on 5% native polyacrylamide gels using 0.5× TBE buffer and visualized using a phosphorimager. The arrows indicate two DNA-bound complexes in (B) and (C). Details of the reactions are given in ‘Materials and Methods’ section. For sequences of the DNA molecules refer to Supplementary Table S1.