Figure 7.

E2 does not induce CDC6 3′-UTR isoform increase in ER-silenced MCF7 cells. Cells were treated with E2 as described earlier. Relative quantification of CDC6 3′-UTR short and long isoforms was determined in MCF7-EV (empty vector transfected), MCF7_CO (control shRNA transfected) and in MCF7_shERα cells before and after 12 h of E2 treatment. The fold change for the isoforms was normalized against the reference gene, SDHA. Quantification was done using the reaction efficiency correction and ΔΔCq method (29). The baseline for the short and long isoforms in untreated samples was set to 1.