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. 2012 Sep 24;40(21):10925–10936. doi: 10.1093/nar/gks882

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Representative measurements of kinetic association rate. (A) The time course of association of Sub-U and WT-RNP. Sub-U (10 nM) was assembled with WT-RNP of 0.125, 0.25, 0.5 or 0.75 μM. FCS was measured over time and the fraction of bound RNA was derived. The curves are the best single-exponential fit, yielding apparent binding rates k_obs. (B) The mean values and SD of k_obs from two measurements are plotted as a function of H/ACA RNP concentration. The line is the best linear fit with the association rate (slope) k_on = 0.0267 ± 0.0005 μM⁻¹ s⁻¹.