Table 2.

Kinetic parameters of reactive substrate and H/ACA RNPs at 27°C

Substrate	H/ACA RNP	koff.S (10−3 s−1)	koff.P (10−3 s−1)	kcat (10−3 s−1)	(10−3 s−1)
Sub-U	WT	n.d.	12 ± 1	n.d.	3.9 ± 0.6
Sub-U	ΔGar1	0.45 ± 0.04	6.4 ± 0.5	0.19 ± 0.05	0.08 ± 0.02

^aEstimated by single-turnover activity assay, n.d., not determined.