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. 2012 Sep 18;40(21):10689–10700. doi: 10.1093/nar/gks857

Figure 1.

Figure 1.

Nonstop, ENY2 and the moiety of Sgf11 form the Drosophila DUBm that interacts with SAGA. (A) Sgf11 structure and homology in different species (Drosophila, yeast and human). White boxes show homologous Sgf11-related domains; dark gray boxes, Sgf11 zinc finger-containing evolutionarily conserved regions and light gray boxes, the SCA7 domain, which is present only in the human protein. (B) Affinity-purified polyclonal antibodies raised in rabbits specifically recognize Sgf11 and Nonstop in nuclear extract from Drosophila embryos in western blot analysis. Lanes were stained with preimmune sera (PI), immune sera (Im) and affinity-purified polyclonal (Ab) antibodies are shown. (C) Sgf11 binds to numerous sites on polytene chromosomes from Drosophila salivary glands. Chromosomes stained with anti-Sgf11 antibodies and DAPI and merged images and scaled-up fragments of chromosomes are shown. (D) Sgf11 interacts with DUBm subunits and the Gcn5 component of SAGA in nuclear extract from Drosophila embryos in co-immunoprecipitation experiments (IP). Antibodies against Sgf11, Nonstop, ENY2 and Gcn5 or preimmune serum (PI) were used. Bands indicated with an asterisk correspond to antibodies.