Skip to main content
. 2012 Sep 18;40(21):10689–10700. doi: 10.1093/nar/gks857

Figure 2.

Figure 2.

Sgf11 is present on the hsp70 promoter region. (A) Sgf11 is present in hsp70 puffs (87 A, 87B) on polytene chromosomes of Drosophila larvae under heat shock. Chromosomes were co-stained with antibodies against Sgf11, Pol II and DAPI. (B) Sgf11 occupancy of the hsp70 promoter increased after heat shock (HS) and significantly decreased after RNase treatment. The presence of Sgf11, Pol II and ENY2 was analyzed by ChIP before (NT) and after heat shock (HS). The results of ChIP are shown as a percentage of input. Here, and in Figures 2C and 6A–G, light gray shading indicates the background (baseline) level determined as the average of measurements in three noncoding sequences (see ‘Materials and Methods’ section). (C) Effects of ENY2 and Nonstop RNAi knockdown on Sgf11 recruitment on the hsp70 promoter under heat shock conditions (HS) according to the results of ChIP (shown as a percentage of input). Cells treated with GFP dsRNA were used as control. (D) The efficiency of ENY2 and Nonstop RNAi knockdown in experiments shown on Figure 3 C as verified by western blot analysis. Cells were treated with GFP dsRNA (control) or dsRNA corresponding to Sgf11 and Nonstop. Tubulin was used as loading control.