Figure 6.

Sgf11 recruitment onto the hsp70 promoter region upon transcription activation depends on Cbp80. (A) Cbp80 occupancy of the hsp70 promoter as studied by ChIP. Chromatin was prepared before heat shock (NT), after heat shock (HS) or after heat shock with RNase treatment (HS+RNase). The results of ChIP are shown as a percentage of input. (B) Specificity of Cbp80 knockdown as tested by western blot analysis. The presence of Cbp80 was analyzed in cells treated with GFP dsRNA (control) or dsRNA. Tubulin was used as loading control. (C–G) The effect of Cbp80 RNAi knockdown on the recruitment of indicated proteins onto the hsp70 promoter after heat shock as studied by ChIP. Western blotting was used to confirm that Cbp80 knockdown did not affect the total level of test proteins in the cells (the results are shown below each figure).