Fig 8.

Yap expression is lost following endocrine specification in the pancreas. (A) Yap is undetectable within glucagon-positive endocrine clusters of the E12.5 mouse pancreas (sequential sections). (B and C) Both Yap and Taz are undetectable within insulin-expressing cells in the E16.5 mouse pancreas. (D) Direct correlation between endocrine specification and mitotic potential within the E12.5 mouse pancreas (the circle denotes a single endocrine cluster). Note cells negative for Ki67 along the length of the prospectively Ngn3-studded E-cadherin-positive trunk region (arrows). (E) Inverse correlation between Ngn3 and Yap expression within the E12.5 mouse pancreas (sequential sections; arrowheads represent individual Ngn3-positive/Yap-negative cells). Scale bar, 50 μm.