Fig 2.

Genetic interactions of the get2Δ deletion with other genes known to influence [PSI⁺] curing. (A and B) Overproduction of Ssa1 (A) or deletion of both SSB genes (ssb1/2Δ) (B) antagonized [PSI⁺] curing by excess Hsp104 in the Get⁺ but not the get2Δ strain, thus revealing an epistatic interaction with the get2Δ deletion. (C to E) Deletion of STI1 (C), CPR7 (D), or UBC4 (E) antagonized [PSI⁺] curing by excess Hsp104 in both the wild-type and get2Δ strains, revealing an additive effect with the get2Δ deletion. In all experiments, cultures were grown in plasmid-selective medium with galactose and raffinose, and aliquots were taken and plated onto plasmid-selective medium with glucose after the specified periods. The presence or absence of [PSI⁺] was detected after replica plating to −Ade medium. At least three experiments were performed for each culture. Standard deviations are shown by error bars. In cases where error bars are not seen, the standard deviation was smaller than the symbol. WT, wild type; control, empty vector.