Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sym1 is imported independent of Tim9/Tim10 and TIM22 complexes. Radiolabeled proteins were imported into mitochondria isolated from the WT and the tim10-2 (A), tim54-11 (B) and tim12-4 (C) mutant strains. In vitro import was performed for the indicated times in the presence or absence of a membrane potential (Δψ). Reisolated mitochondria were solubilized in digitonin and analyzed by BN-PAGE and digital autoradiography.
