Table 1.

Binding parameters derived from isothermal titration calorimetry experiments^a

Parameter (unit)	Value (mean ± SD)
KA (M−1)	2.82 (± 0.16) × 105
KD (μM)	3.54 ± 0.016
ΔH (kcal/mol)	−9.8 ± 0.4
ΔS (cal/mol/°)	−7.35
N (sites)	0.603 ± 0.0206

^aAll experiments were conducted at 30°C. Ligands were placed in the syringe and CLapF in the sample cell. The buffer was 10 mM Tris-HCl–50 mM NaCl₂ adjusted to a pH 7.5 by the addition of concentrated HCl.