Expression levels of PhyaA (A and C) or PhyaA(−50G) (B) fused to lacZ in wild-type, ΔiscR, ΔappY, ΔarcA, ΔappY ΔarcA, ΔiscR ΔappY, ΔiscR ΔarcA, or ΔiscR ΔappY ΔarcA strain backgrounds grown under anaerobic (A and B) or aerobic (C) conditions in MOPS minimal medium containing 0.2% glucose. Strains were assayed for β-galactosidase activity and normalized for cell number, as explained in Materials and Methods. Error bars are for the activity from three strain isolates repeated on at least three independent occasions.