Fig 3.
Expression levels of PhyaA fused to lacZ in strains containing either bla-Ptac-iscR-FRT-cat-FRT-araC-PBAD-iscS (white bars) or bla-Ptac-iscR(C92A)-FRT-cat-FRT-araC-PBAD-iscS (shaded bars) in either a wild-type strain (A and B) or in a strain lacking AppY and ArcA (C). Cells were grown under aerobic (+O2) or anaerobic (−O2) conditions in MOPS minimal medium containing 20 mM arabinose, tetracycline (10 μg/liter), and the indicated concentration of IPTG and were assayed for β-galactosidase activity. Error bars represent data from three independent isolates repeated on at least three independent occasions.