Fig 1.
Circadian expression of aqpZ and effects of deletion of aqpZ on circadian rhythms in Synechocystis. (A) Synechocystis cells containing a bacterial luciferase reporter gene under the control of the aqpZ promoter (PaqpZ::luxAB) were entrained by a 12-h dark period (black box) to synchronize the circadian clock and then shifted to continuous light. The white and gray boxes above the graphs represent subjective day and night, respectively. The PdnaK::luxAB reporter strain was used as a control. The time scale represents the actual time after transfer to continuous light conditions. Data points show means ± SDs from 3 to 48 independent samples. Table 1 summarizes the results. We obtained essentially the same results in three independent experiments. (B) The growth rates of the cells were measured during continuous culture and were calculated on the basis of the optical density of cultures determined every 5 min with an optical sensor.