Fig 8.

Expression of AqpZ protein in Synechocystis cells after hyperosmotic shock. Synechocystis cells were treated with BG11 medium only (A) or with BG11 medium supplemented with 0.5 M sorbitol (B) or 0.5 M NaCl (C). Cell samples taken from the cultures at the indicated times were subjected to Western blot analysis using anti-AqpZ antibodies. The expression level of AqpZ protein was determined by densitometry and is expressed as the level of protein in relation to the level at the beginning of the experiment before addition of sorbitol or NaCl (time zero). Total protein stained with Coomassie brilliant blue R-250 (CBB) served as a loading control.