Table 2.
Riboflavin biosynthesis genes in Streptomyces davawensis
| CDS | Protein and/or function | BLASTP best hit |
|---|---|---|
| BN159_2715 | RibC, bifunctional flavokinase (EC 2.7.1.26) (C-terminal amino acids)/FAD synthetase (EC 2.7.7.2) (N-terminal amino acids) (see reference 17 for experimental proof) | ref|ZP_09958537.1|; bifunctional riboflavin kinase/FMN adenylyltransferase; Streptomyces chartreusis NRRL 12338; identity, 87% |
| BN159_4727 | RibA, GTP cyclohydrolase II (EC:3.5.4.25), monofunctional RibA (see reference 18 for experimental proof) | ref|YP_006246117.1|; YP_006246117.1; unnamed protein product; Streptomyces hygroscopicus subsp. jinggangensis 5008; identity, 90% |
| BN159_4729 | RibG, bifunctional riboflavin specific reductase (EC 1.1.1.193) (N-terminal amino acids)/deaminase (EC 3.5.4.26) (C-terminal amino acids) (see reference 18 for experimental proof) | ref|ZP_06918110.1|; bifunctional enzyme deaminase/reductase; Streptomyces sviceus ATCC 29083; identity, 84% |
| BN159_7144 | RibB, riboflavin synthase (EC 2.5.1.9) (see reference 18 for experimental proof) | ref|NP_625724.1|; NP_625724.1 riboflavin synthase subunit alpha; Streptomyces coelicolor A3(2); identity, 91% |
| BN159_7145 | RibM, riboflavin transporter (see reference 20 for experimental proof) | ref|YP_006244025.1|; YP_006244025.1 unnamed protein product; Streptomyces hygroscopicus subsp. jinggangensis 5008; identity, 85% |
| BN159_7146 | RibA, bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) (N-terminal amino acids)/GTP cyclohydrolase II (C-terminal amino acids) (EC 3.5.4.25), not functional as GTP cyclohydrolase II (see reference 18 for experimental proof) | ref|ZP_06706864.1|; 3,4-dihydroxy-2-butanone-4-phosphate synthase; Streptomyces sp. strain e14; identity, 88% |
| BN159_7147 | RibH, 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78) | ref|ZP_05001584.1|; 6,7-dimethyl-8-ribityllumazine synthase; Streptomyces sp. strain Mg1; identity, 88% |
| BN159_7984 | RibA, monofunctional GTP cyclohydrolase II (EC 3.5.4.25) | gb|EIM43989.1|; bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein; Rhodococcus opacus M213; Length, 381 aa; identity, 51% |
| BN159_7986 | RibB, archaeal riboflavin synthase (EC 2.5.1.9) | ref|YP_003483117.1|; riboflavin synthase; Aciduliprofundum boonei T469; identity, 58% |
| BN159_8007 | RibA, monofunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) | ref|YP_004404734.1|; unnamed protein product; Verrucosispora maris AB-18-032; identity, 74% |
| BN159_0742 | Riboflavin-specific reductase function (EC 1.1.1.193) | Identity of >50% to deaminase-reductase domain-containing proteins |
| BN159_2369 | ||
| BN159_4557 | ||
| BN159_5537 | ||
| BN159_7503 |