Fig 1.

Leucine-independent growth defect of ndgR knockout mutant. (A) Growth comparison of M145 (circles) and BG11 (ΔndgR; triangles) in complex liquid media. R5 sucrose-free media were prepared as described in Materials and Methods. Glucose-medium is marked with closed symbols, while GlcNAc-medium is marked with open symbols. (B) Growth comparison of M145, BG11, and BG22 on three different agar plates. Growth on basic minimal medium (BMM) made of glucose and (NH₄)₂SO₄ was used as a control (None). Growth was compared on BMM supplemented with leucine (+leu) or Casamino Acids (right). WT, wild type.