Fig 3.

Direct interaction of NdgR on target genes. (A) Biosynthetic pathway of methionine. The metF and metH-metE genes tested with DACA are boxed. (B) Gel shift assay. FAM-labeled probe sequences for metH and metF upstream were used. Lane 1, probe only; lane 2, probe with rNdgR; lane 3, rNdgR with labeled and competitor DNAs. ndgR-38 was used as a specific competitor for FAM-metH-48 and FAM-metF-48. (C) Comparison of sco5552 (ndgR) and sco2103 (metF) upstream sequences. Relative position from the translational start codon is indicated, and conserved short motifs are boxed. Asterisks indicate identical residues.