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. 2012 Dec;194(24):6883–6891. doi: 10.1128/JB.01636-12

Fig 1.

Fig 1

Negative regulation of cmeABC by CosR. (A) qRT-PCR analysis of cmeABC transcription. C. jejuni strains were cultured at different CosR levels at 42°C for 8 h under microaerobic conditions to extract total RNA. The CosR-knockdown condition was generated by supplementing 1.5 μM CosR-PNA to the medium. The results of three independent experiments are expressed as means and standard deviations. (B) PcmeABC::lacZ fusion assay in the absence or presence of cholate (CA). Cholate (1 mg/ml) was added to the culture medium, and C. jejuni strains were grown for 6 h prior to the assay. The “–” symbol indicates the cultures without cholate. (C) Binding of rCosR on the cmeABC promoter. Radiolabeled DNA probes of the cmeABC promoter were incubated with rCosR at different concentrations, and unlabeled DNA probes (U.P) and internal coding regions (I.C) of cmeABC were used as a competitor.