Table 2.
Oligonucleotides used in this study
| Primer name | Sequence (5′ to 3′)a | Restriction site |
|---|---|---|
| cBupFor | GCGAAGCTTCGAGATGAAGACGATGTTGTAGCG | EcoRI |
| cBupRev | CGGGAATTCGCGCAGAACAAGATCCTCTCGTTC | HindIII |
| cBdwFor | CGGAAGCTTGCGGTCTTCGTGGTCCTCATC | HindIII |
| cBdwRev | GCGGCATGCACCCACCGAGGAGGCCAGTACC | SphI |
| cMupFor | TCTGAATTCGGTAGTGGACGAGGGTGGCGAC | EcoRI |
| cMupRev | TTATCTAGAGGGATTCGAGAGGATCAGCACG | XbaI |
| cMdwFor | TGTTCTAGACTCCTCATCCGCAACAAGATCGAG | XbaI |
| cMdwRev | TCCAAGCTTCTTTCGACGTGGTCCTCAGCG | HindIII |
| camBColF | GACGACCAGCGCGAGGATGCAG | |
| camBColR | GTCGAAACCCGGAGACGAGGCGTAC | |
| camMColF | ACCAGTAGGGCGCGATCTGATGGAG | |
| camMColR | AGGTGACCCGGCGCGTCAATC | |
| camBpSTf | ATATCTAGAAGACACGTTCGCCGAGTCGGTC | XbaI |
| camBpSTr | AATTCTAGAAGATGTTCGCCAAGACCATCG | XbaI |
| camMpSTf | AAAGAATTCAGACTGAGTGATTCCGTCATGGG | EcoRI |
| camMpSTr | AAATCTAGATGAACACTTGGACTGCCACACCG | XbaI |
| camBpTIPf | CTTGTTACATATGACCATCGTCTGGTCGGGCCTC | NdeI |
| camBpTIPr | ATCAAGCTTTCACACCACCCGTTCCCGCACC | HindIII |
| camBQFor | TCTCGTCCCACACGTTCAAC | |
| camBQRev | CGAAGGTTCCGACCATCATC | |
| camMQFor | CCGTCGCGAAAACATTGG | |
| camMQRev | GGATCGGTCATCACGAACATC |
a
Restriction enzyme cut sites are underlined.