Fig 1.

Alignments of the T. kodakarensis histone sequences (6) using clustalW. Shown are alignments of the amino acid (A) and the encoding nucleic acid (B) sequences of HTkA (TK1413) and HTkB (TK2289). Between the sequences, identical amino acids and nucleotides are indicated by asterisks, and similar amino acids are indicated by colons. The codon changes introduced into TK1413 in plasmid pTS706 to generate plasmids pTS707 and pTS708 are indicated by underlining. An oligonucleotide with the sequence double underlined in TK1413 was synthesized, labeled with ³²P, and used as the probe for Southern blotting (Fig. 2E).