Strains |
|
|
TS517 |
ΔpyrF ΔtrpE::pyrF ΔTK0664 |
33 |
LC124 |
ΔpyrF ΔtrpE::pyrF ΔTK0664 ΔTK1413 |
This study |
LC125 |
ΔpyrF ΔtrpE::pyrF ΔTK0664 ΔTK2289 |
This study |
Plasmids |
|
|
pTS535 |
pUC118 with MCS1-(PTK2279-TK0254 PhmtB-TK0664)-MCS2 |
33 |
pLC113 |
pTS535::TK1412-TK1413-TK0664-TK0254-TK1412-TK1414-TK1415 |
This study |
pLC114 |
pTS535::TK2286-TK2287-TK2288-TK2289-TK0664-TK0254-TK2286-TK2287-TK2288-TK2290 |
This study |
pLC124 |
pTS535::TK1412-TK0664-TK0254-TK1412-TK1414-TK1415 |
This study |
pLC125 |
pTS535::TK2286-TK2287-TK2288-TK0664-TK0254-TK2286-TK2287-TK2288-TK2290 |
This study |
pUDHisD |
pUC118 derivative; ΔhisD::trpE
|
36 |
pTS503 |
pUC118::PhmtB-TK1827-trpE-TK1828 with TAG at codon 5 of TK1827 |
33 |
pLC70 |
PTK2279-TK0254 Pgdh-PF1848 |
32 |
pLC71 |
pLC70 Δp24 |
32 |
pTS414 |
pLC70::PhmtB-rpoL-HA |
32 |
pTS706 |
pLC70::PhmtB-TK1413 |
This study |
pTS707 |
pLC70::PhmtB-TK1413 with TGA at codon 2 of TK1413 (A2STOP) |
This study |
pTS708 |
pLC70::PhmtB-TK1413 with ATT at codon 20 of TK1413 (R20I) |
This study |