Table 3.
Transformation of T. kodakarensis TS517, LC124, and LC125 with replicative and nonreplicative plasmids
| Plasmid | Autonomous replicationa | Gene targetb | Avg no. of transformants recovered ± SDc |
||
|---|---|---|---|---|---|
| TS517 | LC124 (ΔTK1413) | LC125 (ΔTK2289) | |||
| pUDHisD | − | TK0244 | 1 × 104 ± 0.5 × 104 | 0 | 1 × 104 ± 0.5 × 104 |
| pLC124 | − | TK1413 | 1 × 101 ± 1 × 101 | NA | 1 × 101 ± 1 × 101 |
| pLC125 | − | TK2289 | 1 × 104 ± 0.5 × 104 | 0 | NA |
| pTS503 | − | TK1827 | 1 × 104 ± 0.5 × 104 | 0 | 1 × 104 ± 0.5 × 104 |
| pLC70 | + | 2 × 104 ± 1 × 104 | 1 ± 1 | 2 × 104 ± 1 × 104 | |
| pLC71 | + | 2 × 104 ± 1 × 104 | 1 ± 1 | 2 × 104 ± 1 × 104 | |
| pTS414 | + | 2 × 104 ± 1 × 104 | 1 ± 1 | 2 × 104 ± 1 × 104 | |
| pTS706 | + | 0 | 0 | 0 | |
| pTS707 | + | 2 × 104 ± 1 × 104 | 1 ± 1 | 2 × 104 ± 1 × 104 | |
| pTS708 | + | 0 | 0 | 0 | |
a
Plasmid capable (+) or incapable (−) of autonomous replication in T. kodakarensis.
b
Gene that the plasmid was constructed to delete.
c
Transformants recovered after incubation of the T. kodakarensis strain (1012 cells) with 1 μg of the plasmid DNA. NA, not applicable (gene target was already deleted).