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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: Cancer Cell. 2012 Nov 13;22(5):631–644. doi: 10.1016/j.ccr.2012.09.021

Figure 3. PUMA, NOXA and TRB3, but Not Ku70, are Involved in Gln Deprivation-mediated Cell Death.

Figure 3

(A) RT-PCR analysis of genes involved in apoptosis. Data are shown as an average of triplicates.

(B) Western blot analysis of indicated protein levels in Gln-starved Kelly cells.

(C) Viability of Kelly cells transfected with indicated siRNAs in the presence or absence of Gln was analyzed by AnnexinV-PI staining. Data are shown as an average of triplicates.

(D) Examination of Gln starvation-induced cell death in five MYCN-amplified, and four MYCN non-amplified neuroblastoma cell lines. Cell death was measured by Annexin V-PI staining.

(E) Viability of IMR32 or NLF cells transfected with siRNAs targeting PUMA, NOXA, and TRB3 in the presence or absence of Gln was analyzed by AnnexinV-PI staining. Data are shown as an average of triplicates.

(F) Western blots confirming the effect of siRNA knockdown.

All error bars represent standard deviation. *p< 0.01. **p< 0.005. See also Figure S2.