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. Author manuscript; available in PMC: 2012 Nov 30.
Published in final edited form as: J Immunol. 2010 Sep 22;185(8):4705–4713. doi: 10.4049/jimmunol.1002276

Figure 3.

Figure 3

The large size of the 2W:I-Ab-specific population is associated with its specific TCR contact residues. A, I-Ab-binding nonamers for 2W and 3K peptides with TCR and I-Ab contacts indicated. B, Contour plots of CD44 versus 2W:I-Ab (left) or 3K:I-Ab (right) tetramer staining for CD4+ T cells from tetramer-enriched spleen and lymph node samples from naïve B6 mice. C, Total CD4+ 2W:I-Ab+ or 3K:I-Ab+ cells from spleen and lymph nodes (left) or thymus (right) samples of individual B6 mice. Mean values for each group are indicated as horizontal bars and numerically for each set of values. Data are from at least three independent experiments. D, Model structure of 2W:I-Ab (top view). The 2W peptide is in green, while the I-Ab molecule is in blue. The structure is positioned with the peptide N terminus on the left, the I-Ab β1-helix at the bottom and the α1-helix at the top. Black arrows indicate the predicted TCR contact residue side chains. Residue I-Ab β74E that partly occludes residue P5L of the 2W peptide is indicated by a white arrow.