Fig. 4.

Suppression of fERG or ILK expression disrupts Matrigel invasion of fERG-PrECs. (A) fERG-BPH-1 and –RWPE-1 cells were transfected with ERG- or ILK-targeted siRNA as described in Materials and methods for 2 days and lysates were immunoblotted for respective ILK and ERG expression using β-actin immunoblotting as a loading control. (B) Cells siRNA transfected as in A were plated onto Matrigel-coated transwell chambers and assayed for invasion after 1 day. Representative images of bottom of transwell chamber are provided, Scale bar, 100 µm. (C) Quantification of invasion assays of respective Mock and fERG-PrECs expressed as average number of migrated cells per field in six random fields ± standard error of the mean (*different than untreated fERG, P < 0.01, †different than Mock, P < 0.01).