FIGURE 6.

Transcriptional alternations induced by ^SG and S⁶mG in 293T cells treated with either or both CSB and XPC siRNAs. A, representative gel images showing the restriction fragments released from the RT-PCR products arising from transcription templates containing ^SG (lanes 6, 9, 12, and 15), S⁶mG (lanes 7, 10, 13, and 16), or normal guanine (G, lanes 8, 11, 14, and 17). 10mer-TA, 10mer-TG, 10mer-TT, and 10mer-TC represent standard ODNs d(CTAGNTTTGC), where N is A, C, G, and T, respectively (lanes 1–4, respectively). 13mer-Comp represents standard ODN d(CATCAAGCTTTGC) (lane 5), which corresponds to the restriction fragment arising from the competitor genome. The band observed between 13mer-Comp and 10mer-TT is most likely a nonspecific digestion product and is not lesion-dependent, because it is also obtained with lesion-free control templates. B and C, the RMF values of S⁶mG (B) and the RBE values of ^SG and S⁶mG (C) in 293T cells treated with either or both CSB and XPC siRNAs. The data represent the means and standard error of results from three independent experiments. *, p < 0.05; **, p < 0.01. The p values were calculated by using unpaired two-tailed Student's t test.