FIGURE 6.
LOX activity is required for B2R but not for MRGPR-X1-promoted TRPV1 activation. Ca2+ signals in single fura2-loaded F11-MRGPR-X1 or F11-MRGPR-X1/TRPV1 cells were monitored. BK (100 nm, final) (A and B) and BAM8–22 (2 μm, final) (C and D) were manually injected at time point 50 s. Cells were preincubated with ETOH (0.1%, 30 min) (A and C) and NDGA (10 μm, 30 min) (B and D). Data from 3–5 independent experiments were compiled, normalized by defining the first ratio (0.5 s) measured as 100%, and expressed as the mean ± S.E. The AUC for each measured cell was determined, and data of all cells were compiled, expressed as the mean ± S.E. and presented in bar graphs. Asterisks indicate a significant difference (***, p < 0.001; **, p < 0.01) between F11-MRGPR-X1/TRPV1 and F11-MRGPR-X1 cells; hash signs indicate a significant difference (###, p < 0.001) between ETOH- and NDGA-treated F11-MRGPR-X1/TRPV1 cells stimulated with BK.