FIGURE 8.

DAG/OAG-induced TRPV1 activation in F11 cells. Ca²⁺ signals in fura2-loaded F11-MRGPR-X1 or F11-MRGPR-X1/TRPV1 cells were monitored. DAG kinase (DAGK) inhibitor-2 (10 μm, final) (A) and OAG (100 μm, final) (B and C) were manually injected at time point 50 s. Cells were preincubated with BIM-X (1 μm, 30 min) (A and C) and a separate pool of cells with the carrier DMSO (0.1%, 30 min) (C). Data from 3–5 independent experiments were compiled, normalized by defining the first value (0.5 s) measured as 100%, and expressed as the mean ± S.E. The AUC for each measured cell was determined, and data of all cells were compiled, expressed as the mean ± S.E., and presented in bar graphs. Asterisks indicate a significant difference (***, p < 0.001) between F11-MRGPR-X1/TRPV1 and F11-MRGPR-X1 cells; hash signs indicate a significant difference (###, p < 0.001) between BIM-X- and DMSO-treated F11-MRGPR-X1/TRPV1 cells.