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Competition of sSMRT and NCoR during GR-mediated induction of GREtkLUC in U2OS cells. Competition assays were conducted and analyzed as in Fig. 1 with the indicated amounts of sSMRT and NCoR plasmids plus 0.2 ng of GR and 100 ng of GREtkLUC plasmids. A and B, shown are plots of Amax/EC50 versus NCoR and 1/EC50 versus NCoR, respectively, in competition of NCoR versus sSMRT. Graphs shown are for a single representative experiment (total = 3).
