FIGURE 5.

Inhibition of Certhrax glycohydrolase activity. Shown are Lineweaver-Burk plots for Certhrax in the presence of various concentrations of inhibitors PJ34 (A) or P6F (B). The glycohydrolase activity of Certhrax was measured with 0 (circles), 6 (squares), 12 (diamonds), and 24 (triangles) μm inhibitor. v_o indicates initial velocity. Error bars, S.E. C, inhibitor P6 protection from cytotoxicity of Certhrax-intoxicated cells as determined by half-maximal effective concentration (EC₅₀). RAW264.7 cells were exposed to a lethal dose of Certhrax (10 ng/ml, 500 times LD₅₀) at varying concentrations of compound P6. Error bars, S.D.