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Kinetic parameters of Certhrax glycohydrolase activity
Kinetic parameters were determined as described under “Experimental Procedures.” All measurements represent the mean ± S.E. of at least six replicates, from at least three independent experiments.
| Parameter | Certhrax |
|---|---|
| Km (μm) | 42 ± 11 |
| kcat (min−1) | 8.7 ± 1.0 |
| kcat/Km (m−1 min−1) | 2.1 × 105 |
| KD(NAD+) (μm) | 52 ± 12 |
