TABLE 3.
Strain | Porina, P | Secretionb |
E1c |
E2d |
|||||
---|---|---|---|---|---|---|---|---|---|
Km | Vmax | k1 | k−1 | k1 | k−1 | k2 | k−2 | ||
cm s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | |||
Wild type | 1.5 × 10−6 | 170 ± 10 μm | 420 ± 20 nm s−1 | (12 ± 1) × 10−4 | (20 ± 5) × 10−4 | (15 ± 2) × 10−4 | (9.7 ± 0.6) × 10−4 | (18 ± 3) × 10−4 | (34 ± 3) × 10−4 |
AFS-I-hgdD | NDe | 40 ± 8 m | 25 ± 5 mm s−1 | (14 ± 4) × 10−4 | (0.8 ± 0.2) × 10−4 | (19 ± 2) × 10−4 | (6 ± 1) × 10−6f | (15 ± 5) × 10−4 | (27 ± 5) × 10−4 |
AFS-I-hgdC | ND | ND | ND | ND | ND | (18 ± 2) × 10−4 | (6.9 ± 0.9) × 10−4 | (13 ± 2) × 10−4 | (35 ± 3) × 10−4 |
CSCW2 | ND | ND | ND | (9 ± 2) × 10−4 | (31 ± 9) × 10−4 | (18 ± 4) × 10−4 | (15 ± 6) × 10−4g | (18 ± 8) × 10−4 | (5 ± 1) × 10−4f |
AFS-I-all2215 | ND | ND | ND | (13 ± 2) × 10−4 | (24 ± 7) × 10−4d | (19 ± 5) × 10−4 | (14 ± 5) × 10−4g | (11 ± 4) × 10−4 | (2 ± 1) × 10−4f |
AFS-I-anaOmp85 | ND | ND | ND | ND | ND | (3.7 ± 0.6) × 10−4f | (7 ± 2) × 10−4 | (19 ± 6) × 10−4 | (25 ± 7) × 10−4 |