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. 2012 Oct 15;287(49):41126–41138. doi: 10.1074/jbc.M112.396010

TABLE 3.

Kinetic parameters determined in this study

Strain Porina, P Secretionb
E1c
E2d
Km Vmax k1 k−1 k1 k−1 k2 k−2
cm s1 s1 s1 s1 s1 s1 s1
Wild type 1.5 × 10−6 170 ± 10 μm 420 ± 20 nm s−1 (12 ± 1) × 10−4 (20 ± 5) × 10−4 (15 ± 2) × 10−4 (9.7 ± 0.6) × 10−4 (18 ± 3) × 10−4 (34 ± 3) × 10−4
AFS-I-hgdD NDe 40 ± 8 m 25 ± 5 mm s−1 (14 ± 4) × 10−4 (0.8 ± 0.2) × 10−4 (19 ± 2) × 10−4 (6 ± 1) × 10−6f (15 ± 5) × 10−4 (27 ± 5) × 10−4
AFS-I-hgdC ND ND ND ND ND (18 ± 2) × 10−4 (6.9 ± 0.9) × 10−4 (13 ± 2) × 10−4 (35 ± 3) × 10−4
CSCW2 ND ND ND (9 ± 2) × 10−4 (31 ± 9) × 10−4 (18 ± 4) × 10−4 (15 ± 6) × 10−4g (18 ± 8) × 10−4 (5 ± 1) × 10−4f
AFS-I-all2215 ND ND ND (13 ± 2) × 10−4 (24 ± 7) × 10−4d (19 ± 5) × 10−4 (14 ± 5) × 10−4g (11 ± 4) × 10−4 (2 ± 1) × 10−4f
AFS-I-anaOmp85 ND ND ND ND ND (3.7 ± 0.6) × 10−4f (7 ± 2) × 10−4 (19 ± 6) × 10−4 (25 ± 7) × 10−4

a See Fig. 3.

b See Fig. 6.

c See Figs. 3 and 11.

d See Fig. 11.

e ND, not determined.

f Significance of change compared with wild type with p < 0.005.

g Significance of change compared with wild type with p < 0.01.