FIGURE 4.

Activity of H2 analogues with truncations and/or single alanine mutation on the RXFP2 receptor. A, competition binding results of native H2 relaxin and H2 analogues with truncation and/or single alanine mutation in the presence of a competitive ligand Eu³⁺-labeled INSL3 tested in HEK-293T cells stably expressing RXFP2 receptors. Data are expressed as a percentage of specific binding and are pooled data from at least three experiments performed in triplicate. B, cAMP activity of native H2 relaxin and H2 analogues with truncation and/or single alanine mutation in HEK-293T cells expressing RXFP2 receptors using a pCRE-galactosidase reporter gene system. Data are expressed as a percentage of maximum INSL3-stimulated cAMP response and are pooled data from at least three experiments performed in triplicate. Error bars, S.E.