FIGURE 5.

Activity of chimeric H2/INSL5 A-chains on the RXFP1 receptor. A, competition binding results of native H2 relaxin and chimeric H2/I5 A-chain relaxin analogues in the presence of a competitive ligand Eu³⁺-labeled H2 relaxin tested in HEK-293T cells stably expressing RXFP1 receptors. Data are expressed as a percentage of specific binding and are pooled data from at least three experiments performed in triplicate. B, cAMP activity of native H2 relaxin and H2 analogues with truncation and/or single alanine mutation in HEK-293T cells expressing RXFP1 receptors using a pCRE-galactosidase reporter gene system. Data are expressed as a percentage of maximum H2 relaxin-stimulated cAMP response and are pooled data from at least three experiments performed in triplicate. Error bars, S.E.