TABLE 1.

Potency and selectivity of endogenous SCFAs ligands at human and mouse orthologs of FFA2 and FFA3

NR, no response.

	Human			Mouse			Ortholog selectivitya
	hFFA2b	hFFA3b	Selectc	mFFA2b	mFFA3b	Selectc	FFA2	FFA3
C1	2.99 ± 0.23	NR		2.17 ± 0.43	2.28 ± 0.34	−0.11	0.82
	(50 ± 7)			(50 ± 24)	(91 ± 30)
C2	4.45 ± 0.09	2.68 ± 0.18	1.77	3.55 ± 0.11	3.73 ± 0.20	−0.18	0.90	−1.05
	(99 ± 3)	(78 ± 10)		(96 ± 5)	(87 ± 8)
C3	4.22 ± 0.08	4.09 ± 0.08	0.13	3.73 ± 0.09	4.87 ± 0.12	−1.14	0.49	−0.78
	(100)	(100)		(100)	(100)
C4	4.26 ± 0.10	4.02 ± 0.10	0.24	3.47 ± 0.12	3.67 ± 0.17	−0.2	0.79	0.35
	(105 ± 6)	(66 ± 3)		(104 ± 6)	(98 ± 8)

^a Ortholog selectivity represents: human pEC₅₀ − mouse pEC₅₀.

^b pEC₅₀ values are reported with E_max expressed as a percentage of the C3 response in parenthesis.

^c Selectivity represents comparisons within species of: FFA2 pEC₅₀ − FFA3 pEC₅₀.