FIGURE 2.

HPKO platelets display abnormal platelet spreading in the absence of secretory inhibitors. WT and HPKO mouse platelets (5 × 10⁷ platelets/ml) were spread for up to 90 min on the fibrinogen-coated coverslips. A, immunofluorescence analysis was performed using Phalloidin-Alexa 594 to detect F-actin. Scale bar, 10 μm. Images are representative of three separate experiments. B, adhesion was determined by random counts of platelet number per field of view (n = 10). C, bar graphs display cell surface area (mean ± S.E.) of WT and HPKO platelets using the Metamorph® software. Error bars, S.E. from three separate experiments. *, p < 0.05.