TABLE 1.
PtdInsP selectivity of protrudin-FYVE determined by equilibrium SPR measurements
| Proteins |
Kda |
|||||
|---|---|---|---|---|---|---|
| PtdIns(3,4,5)P3 | PtdIns(4,5)P2 | PtdIns(3,4)P2 | PtdIns(3)P | PtdIns(4)P | PtdIns(5)P | |
| nm | ||||||
| WT | 190 ± 30 | 180 ± 20 | 125 ± 20 | >2000b | >2000 | >2000 |
| 4A mutant | >2000 | >2000 | >2000 | NMc | NM | NM |
| K367A/R369A | >2000 | >2000 | >2000 | NM | NM | NM |
a POPC/POPS/PtdInsP (77:20:3 in mol %) vesicles were used for Kd determination.
b Showing a binding signal that does not, however, approach saturation with the protein concentration up to 2 μm.
c Not measured.