FIGURE 1.

p426-ATMdexa1 and p426-MetMet His-tagged constructs. A, the entire ATMdexa1 cDNA was cloned into p426 expression vector carrying N-terminal and C-terminal His tags. Translation of the transcribed mRNA can potentially generate two recombinant proteins: an N terminus His-tagged protein starting from vector ATG codon (first 90 amino acids of the native protein, colored in red) and a C terminus His-tagged protein from a putative start codon following position 825 (maximum 252 amino acids, colored in blue). B, the second half of ATMdexa1 cDNA (825–1580 residues) was cloned into the p426 expression vector, generating a double-tagged recombinant protein (N terminus and C terminus His tags) of 252 amino acids.