FIGURE 6.

Functionality of miniATM. A, yeast complementation assay was performed with tel1Δ (KSC1368) and mec1-81tel1Δ (KSC1402) yeast mutants transfected with pYES2-ATMdexa1 or empty pYES2 constructs. Recombinants were grown on solid selective medium or liquid selective medium supplemented with the DSB inducer phleomycin (B) to assess the ability of ATMdexa1 to complement TEL1 and TEL1-MEC1 deficiencies. O.D., optical density. C, miniATM kinase assay. Recombinant GST-miniATM was tested for in vitro phosphorylation of PHAS-I substrate in the presence of [γ-³²P]ATP. Two kinase concentrations were tested, with or without wortmannin inhibition.